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Statistical analysis indicated that P. rufus infections were not strongly age-dependent, as no statistically significant difference was found between the positive and negative strata of sex-weighted cumulative FOI estimates (Fig. 5 a; Additional file 3 : Text S5, Table S9; all pairwise comparisons P > 0.05). Bats positive for babesial infection were also found to have a statistically significantly higher age at recovery than those carrying Babesia spp. strains (Fig. 5 b; Additional file 3 : Text S5, Table S9; P = 0.018). Age at recovery was within the range of 1–6 years (Additional file 3 : Text S6).
In order to determine if our results from surveillance differed from those produced by the International Atomic Energy Agency (IAEA), we compared the results of our targeted surveillance for babesial infections against the IAEA database. Of our 202 P. rufus bats, 194 (95.15%) were present at one of the 22 focal locations sampled by IAEA, of which 46 (23.6%) P. rufus bats were positive for Babesia spp. and P. rufus infections were present in only two of the six IAEA locations sampled (Table S10). The discrepancy between our results and those from the IAEA is likely attributable to differences in the IAEA’s sampling techniques as opposed to specific sampling locations or diurnal site differences. The IAEA primarily focuses on IAEA-proscribed sites during a three-week pre-dawn to dusk sampling period from August to October, and all sites are sampled using the same trapping technique (tape-cloth trapping). Additionally, the median sample size per site sampled by the IAEA for 2015 was only three (range 1-11). Our P. rufus samples were collected during November 2013–January 2016 using a one-night sampling approach and it is likely that the limited collection across a period of two months resulted in sample over-representation of a specific locality (n = 194) and seasonal fluctuations. An additional caveat is that not all IAEA-sampled locations carry out trappings within 15 days of the IAEA’s monthly pre-dawn to dusk sampling period. Therefore, we cannot exclude the possibility that we misclassified infected bats as P. rufus-negative due to sampling outside of the IAEA pre-dawn to dusk period. Despite these caveats, our sample size was sufficient to detect age-related Babesia spp. infections. Additionally, our total sample size of 176 did not differ greatly from the 191 bats captured at the IAEA-sampled locations (Additional file 1 : Table S1), thus indicating that our sample adequately represents IAEA sampling of the region in the year 2015.
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Rufus Wilde is a bounty hunter who wields his trusty dual pistol, the Earteeth. He is best known for his mid-range prowess and pretty flashy and damaging moves. His Make it Rain ability, for example, is where he shoots upward, and thousands of bullets rain down onto an area around him, dealing massive damage.
Ah, yes, Rufus.
We know you from your days in the ‘70s funk band Chaka Khan And Rufus, and we know you’ve been doing incredible things since then, too. You wrote the theme song for the movie The Guyana-Dakota War, and you’ve been on tour with your new band Rhinogr since 2015, but today we have an even bigger treat than you knew you were going to get when you signed up for the YouTube Music Awards.
Hometown sounds is the name of the game when it comes to reuniting with the past, which is why today we’re looking back at a favorite album by an iconic Chicago funk band who got it all going with their self-titled, Cracked Rufus Download And Chaka Khan album, and a record that’s so good that it’s probably the reason why Chaka Khan has lasted through all these years.
Rufus can be found lurking in the shadowy alleys and tunnels of Capital Wasteland sometimes doing some vigilante work. He can also be found sifting through trash bins or sleep-deprived at the local diner. Come and see why he has such a bad habit of chasing down thieves and slackers.
1. Born in Chicago, Rufus grew up in Santa Rosa, California, and Newark, New Jersey.
2. The scientific methods and disciplines that Rufus pursues are Biochemistry, Molecular Biophysics, Plant Biology, and Nutritional Sciences.
3. The work of Rufus and his peers is used by researchers to address questions related to human and animal nutrition and metabolism.
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Of all the stories in the wide world, Rufus has chosen to be a flying-winged, cat-dog creature. In our world, we’d call such a being a fairy. In his world, his home realm of All-In, he is a being of several parts — and none of them are what you’d find in a domestic pet. His body is composed of four heads which he can use as legs, paws, wings, and antennas. He also has a tail. And because he’s not a real pet, he can also turn himself into a massive lizard. Probably there are others in his world who are more familiar with Rufus than we are. With his appearance, his personality, and his many distinct behaviors, the movie character resembles The Rufus, otherwise known as Miao Yin, of the Chinese classic novelJourney to the West. The Rufus who speaks English, however, is simply named “Rufus” — no flower petals or rope-knots involved.
It turns out Rufus was searching for his people’s dust, which is not the kind of thing you’d casually buy for a pet and take home to your family. He wants to go home. The kids need to make sure it doesn’t fall into the wrong hands. It isn’t until Rufus is in danger of perishing on the island that Cory Phillips’s character has a dramatic personality change. His character is much more heroic than the noisy, clunky, and dangerous mob of villains. And he’s still voiced by the amazing Ryan Bellgardt.
With Rufus, top artists from around the world come together to provide a genuine glimpse into what they might create if they were given complete artistic control over an idea. From its opening sequence, this short film uses a few dazzling animation experiments to create a world that is very different from our own, while also giving a sense of immediacy and magic to what might be a fantastic world.
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What’s new in Rufus
- new boss enemy: He is a bit faster than previous Rufus, but otherwise the fight is similar
- new moveset: Lots of ground attacks, but also a number of different anime style attacks
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- 2 GB of free space for the ISO files.
- Minimal speed of your USB flash drive >10 MB/sec.
- A computer with BIOS boot mode >2.2
- A DVD/USB drive.
- Additional space on the same USB flash drive >1 GB (2 GB if using the Offline system).
- A full-function keyboard and mouse.
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